Peptide-coupled alginate

Peptide Coupled Alginates

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NOVATACH™ series of peptide-coupled alginates are designed to facilitate and promote interaction between cells and alginate-based scaffolds and matrices and have utility in formulations where cell-to-matrix interaction is beneficial.
NOVATACH™ peptide-coupled alginates have been modified using aqueous carbodiimide chemistry to covalently graft peptide sequences to alginate molecules [2]. Cell interaction with biomaterials is mediated through transmembrane receptors that recognize adhesion molecules at material surfaces. The RGD peptide sequence is a common example of such adhesion ligand, and it has been shown that RGD-alginates successfully initiate biological interactions between alginate hydrogels and cells.
NOVATACH™ peptide-coupled alginates are classified as research products, and are not intended for human use.

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Successful engineering of tissue constructs may one day allow routine replacement of damaged organs and tissues. Alginates are well established as matrix materials for cells or tissue within regenerative medicine and have a promising potential in a variety of biomedical applications [1]. Alginates are naturally-derived polysaccharides composed of (1-4)-linked β-D-mannuronate (M-units) and α-L-guluronate (G-units) monomers, which vary in amount and sequential distribution along the polymer chain depending on the source of the alginate. Alginates suitable for biomedical use is today commercially available from NovaMatrix under the tradename PRONOVA™.
NOVATACH™ is a series of peptide-coupled alginates which facilitate and promote interaction between cells and alginate-based scaffolds and matrices resulting in improved cell functionality and viability. NOVATACH™ extends the range of high quality, well documented, ultrapure and low endotoxin PRONOVA™ alginates for cell therapy and tissue engineering applications and has utility in formulations where cell-to-matrix interaction is beneficial.

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Figure 1: RGD-alginate improves in-vivo cartilage formation in mice. Bovine Articular Chondrocytes (BAC) grown in alginate gives an inhomogeneous and loose structure (left). BAC grown in RGD-alginate gives a homogeneous and firm structure (middle), and displays significantly higher growth rate than BAC in alginate alone (right). Data from Alsberg et al. [3].

NOVATACH™ peptide-coupled alginates have been modified using aqueous carbodiimide chemistry to covalently graft peptide sequences to alginate molecules [2]. Cell interaction with biomaterials is mediated through transmembrane receptors that recognize adhesion molecules at material surfaces. The RGD peptide sequence is a common example of such adhesion ligand and it has been shown that RGD-alginates successfully initiate biological interactions between alginate hydrogels and cells [2,3].

References:

[1] J.E. Melvik, M. Dornish, Alginate as a carrier for cell immobilisation, Book Chapter: Fundamentals of cell Immobilisation Biotechnology (2004), ISBN 1-4020-1887-8, 33-51.

[2] J.A. Rowley, G. Madlambayan, D.J. Mooney, Alginate hydrogels as synthetic extracellular matrix materials, Biomaterials 20 (1999), 45-53.

[3] E. Alsberg, K.W. Anderson, A. Albeiruti, J.A. Rowley, D.J. Mooney, Engineering growing tissues, PNAS 99 (2002), 12025-12030.

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NOVATACH™ peptide-coupled alginates are available in vials containing 100 mgs of sterile-filtered and lyophilized material. The following peptide sequences are currently available:

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G/M Ratio